MED1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MED1.
Immunogen
A synthetic peptide corresponding to human MED1.
Host
Rabbit
Theoretical MW (kDa)
170
Reactivity
Human, Mouse
Specificity
MED1 polyclonal antibody detects endogenous levels of MED1 protein.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat cell lysate with MED1 polyclonal antibody (Cat # PAB26869).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using MED1 polyclonal antibody (Cat # PAB26869). -
Gene Info — MED1
Entrez GeneID
5469Protein Accession#
Q15648Gene Name
MED1
Gene Alias
CRSP1, CRSP200, DRIP205, DRIP230, MGC71488, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2
Gene Description
mediator complex subunit 1
Omim ID
604311Gene Ontology
HyperlinkGene Summary
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq
Other Designations
PPAR binding protein|PPARG binding protein|thyroid hormone receptor-associated protein complex component TRAP220|thyroid receptor interacting protein 2|vitamin D receptor-interacting protein 230 kD|vitamin D receptor-interacting protein complex component
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Interactome
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Disease
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