ERN2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of lysate from HT-29 cell line, using ERN2 Antibody (N-term)(Cat # PAB2675). Diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35 ug.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ERN2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ERN2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of lysate from HT-29 cell line, using ERN2 Antibody (N-term)(Cat # PAB2675). Diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35 ug. -
Gene Info — ERN2
Entrez GeneID
10595Protein Accession#
NP_150296;Q76MJ5Gene Name
ERN2
Gene Alias
IRE1b
Gene Description
endoplasmic reticulum to nucleus signaling 2
Omim ID
604034Gene Ontology
HyperlinkGene Summary
S. cerevisiae
Other Designations
ER to nucleus signalling 2|IRE1 beta|IRE1, S. cerevisiae, homolog of|endoplasmic reticulum to nucleus signalling 2|inositol-requiring 1 beta
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Interactome
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Disease
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Publication Reference
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Translational control by the ER transmembrane kinase/ribonuclease IRE1 under ER stress.
Iwawaki T, Hosoda A, Okuda T, Kamigori Y, Nomura-Furuwatari C, Kimata Y, Tsuru A, Kohno K.
Nature Cell Biology 2001 Feb; 3(2):158.
Application：IP-WB, Human, HeLa cells.
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A role for presenilin-1 in nuclear accumulation of Ire1 fragments and induction of the mammalian unfolded protein response.
Niwa M, Sidrauski C, Kaufman RJ, Walter P.
Cell 1999 Dec; 99(7):691.
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Cloning of mammalian Ire1 reveals diversity in the ER stress responses.
Wang XZ, Harding HP, Zhang Y, Jolicoeur EM, Kuroda M, Ron D.
The EMBO Journal 1998 Oct; 17(19):5708.
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Translational control by the ER transmembrane kinase/ribonuclease IRE1 under ER stress.
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