G3BP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells treated with IL-4 (100 ug/mL, 30 min) using G3BP1 polyclonal antibody (Cat # PAB26683).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Imunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP1 polyclonal antibody (Cat # PAB26683).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of G3BP1.
Immunogen
A synthetic peptide corresponding to residues surrounding S232 of human G3BP1.
Sequence
S-S-Sp-P-A
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells treated with IL-4 (100 ug/mL, 30 min) using G3BP1 polyclonal antibody (Cat # PAB26683).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Imunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP1 polyclonal antibody (Cat # PAB26683). -
Gene Info — G3BP1
Entrez GeneID
10146Protein Accession#
Q13283Gene Name
G3BP1
Gene Alias
G3BP, HDH-VIII, MGC111040
Gene Description
GTPase activating protein (SH3 domain) binding protein 1
Omim ID
608431Gene Ontology
HyperlinkGene Summary
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq
Other Designations
GAP binding protein|OTTHUMP00000160618|Ras-GTPase-activating protein SH3-domain-binding protein|RasGAP-associated endoribonuclease G3BP
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Interactome
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