BCKDK polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of BCKDK (arrow) using BCKDK polyclonal antibody (Cat # PAB2655). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCKDK gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with BCKDK polyclonal antibody (Cat # PAB2655) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BCKDK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human BCKDK.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of BCKDK (arrow) using BCKDK polyclonal antibody (Cat # PAB2655). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCKDK gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with BCKDK polyclonal antibody (Cat # PAB2655) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — BCKDK
Entrez GeneID
10295Protein Accession#
NP_005872;O14874Gene Name
BCKDK
Gene Alias
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Gene Description
branched chain ketoacid dehydrogenase kinase
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000044999|branched chain alpha-ketoacid dehydrogenase kinase
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Interactome
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Disease
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Publication Reference
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Branched-chain alpha-ketoacid dehydrogenase kinase. Molecular cloning, expression, and sequence similarity with histidine protein kinases.
Popov KM, Zhao Y, Shimomura Y, Kuntz MJ, Harris RA.
The Journal of Biological Chemistry 1992 Jul; 267(19):13127.
Application：WB-Re, WB-Ti, Rat, Rat hearts, Recombinant protein.
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Regional assignment of two genes of the human branched-chain alpha-keto acid dehydrogenase complex: the E1 beta gene (BCKDHB) to chromosome 6p21-22 and the E2 gene (DBT) to chromosome 1p31.
Zneimer SM, Lau KS, Eddy RL, Shows TB, Chuang JL, Chuang DT, Cox RP.
Genomics 1991 Jul; 10(3):740.
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Branched-chain alpha-ketoacid dehydrogenase kinase. Molecular cloning, expression, and sequence similarity with histidine protein kinases.
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