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MAPKAPK-2 (phospho T334) polyclonal antibody

  • Catalog # : PAB25880
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAPKAPK-2.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T334 of human MAPKAPK-2.
  • Sequence:
  • P-Q-Tp-P-L
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 49
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Affinity chromatography
  • Concentration:
  • 1 mg/mL
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extracts from HeLa cells treated with UV (20 min) using MAPKAPK-2 (phospho T334) polyclonal antibody (Cat # PAB25880).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK-2 (phospho T334) polyclonal antibody (Cat # PAB25880).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 9261
  • Gene Name:
  • MAPKAPK2
  • Gene Alias:
  • MK2
  • Gene Description:
  • mitogen-activated protein kinase-activated protein kinase 2
  • Gene Summary:
  • This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000034531,OTTHUMP00000034532
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