SEMA6A polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of SEMA6A polyclonal antibody (Cat # PAB2588) pre-incubated with (right lane) and without (left lane) blocking peptide in mouse cerebellum tissue lysate. SEMA6A (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brainreacted with SEMA6A polyclonal antibody (Cat # PAB2588), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SEMA6A.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human SEMA6A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of SEMA6A polyclonal antibody (Cat # PAB2588) pre-incubated with (right lane) and without (left lane) blocking peptide in mouse cerebellum tissue lysate. SEMA6A (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brainreacted with SEMA6A polyclonal antibody (Cat # PAB2588), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — SEMA6A
Entrez GeneID
57556Protein Accession#
NP_065847;Q9H2E6Gene Name
SEMA6A
Gene Alias
HT018, KIAA1368, SEMA, SEMA6A1, SEMAQ, VIA
Gene Description
sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
Omim ID
605885Gene Ontology
HyperlinkGene Summary
sema domain
Other Designations
sema VIa|semaphorin 6A-1
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Interactome
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Pathway
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Disease
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Publication Reference
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From plasma membrane to cytoskeleton: a novel function for semaphorin 6A.
Prislei S, Mozzetti S, Filippetti F, De Donato M, Raspaglio G, Cicchillitti L, Scambia G, Ferlini C.
Molecular Cancer Therapeutics 2008 Jan; 7(1):233.
Application：WB-Ce, Human, A2780 cells.
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Comparative integromics on non-canonical WNT or planar cell polarity signaling molecules: transcriptional mechanism of PTK7 in colorectal cancer and that of SEMA6A in undifferentiated ES cells.
Katoh M, Katoh M.
International Journal of Molecular Medicine 2007 Sep; 20(3):405.
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The class 6 semaphorin SEMA6A is induced by interferon-gamma and defines an activation status of langerhans cells observed in pathological situations.
Gautier G, de Saint-Vis B, Senechal B, Pin JJ, Bates EE, Caux C, Geissmann F, Garrone P.
The American Journal of Pathology 2006 Feb; 168(2):453.
Application：IHC-Fr, Flow Cyt, Human, Human dendritic cells, Human skin.
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From plasma membrane to cytoskeleton: a novel function for semaphorin 6A.
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