PRKCD (phospho S645) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from MCF7 using PRKCD (phospho S645) polyclonal antibody (Cat # PAB25874).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PRKCD (phospho S645) polyclonal antibody (Cat # PAB25874).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKCD.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S645 of human PRKCD.
Sequence
R-L-Sp-Y-S
Host
Rabbit
Theoretical MW (kDa)
78
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography
Concentration
1 mg/mL
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from MCF7 using PRKCD (phospho S645) polyclonal antibody (Cat # PAB25874).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PRKCD (phospho S645) polyclonal antibody (Cat # PAB25874). -
Gene Info — PRKCD
Entrez GeneID
5580Protein Accession#
Q05655Gene Name
PRKCD
Gene Alias
MAY1, MGC49908, PKCD, nPKC-delta
Gene Description
protein kinase C, delta
Omim ID
176977Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
protein kinase C delta VIII
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Interactome
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Pathway
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Disease
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