VIM polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of VIM (arrow) using rabbit VIM polyclonal antibody (Cat # PAB2587). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinomareacted with VIM polyclonal antibody (Cat # PAB2587), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of VIM.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S82 of human VIM.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of VIM (arrow) using rabbit VIM polyclonal antibody (Cat # PAB2587). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinomareacted with VIM polyclonal antibody (Cat # PAB2587), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — VIM
Entrez GeneID
7431Protein Accession#
NP_003371;P08670Gene Name
VIM
Gene Alias
FLJ36605
Gene Description
vimentin
Omim ID
193060Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Other Designations
OTTHUMP00000019224
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Interactome
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Disease
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Publication Reference
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Vimentin filaments support extension of tubulin-based microtentacles in detached breast tumor cells.
Whipple RA, Balzer EM, Cho EH, Matrone MA, Yoon JR, Martin SS.
Cancer Research 2008 Jul; 68(14):5678.
Application：IF, Human, MCF10A, MDA-436 cells.
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Interaction of surfactant protein A with the intermediate filaments desmin and vimentin.
Garcia-Verdugo I, Synguelakis M, Degrouard J, Franco CA, Valot B, Zivy M, Chaby R, Tanfin Z.
Biochemistry 2008 May; 47(18):5127.
Application：WB, Human, U-937 cells.
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Filensin: a new vimentin-binding, polymerization-competent, and membrane-associated protein of the lens fiber cell.
Merdes A, Brunkener M, Horstmann H, Georgatos SD.
The Journal of Cell Biology 1991 Oct; 115(2):397.
Application：IEM, Pig, Pig eyes.
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Vimentin filaments support extension of tubulin-based microtentacles in detached breast tumor cells.
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