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EIF2S1 (phospho S51) polyclonal antibody

  • Catalog # : PAB25861
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of EIF2S1.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S51 of human EIF2S1.
  • Sequence:
  • E-L-Sp-R-R
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 38
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Affinity chromatography
  • Concentration:
  • 1 mg/mL
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extracts from K-562 cells untreated or treated with IFN-alpha (100 ng/mL 20 min) using EIF2S1 (phospho S51) polyclonal antibody (Cat # PAB25861).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EIF2S1 (phospho S51) polyclonal antibody (Cat # PAB25861).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1965
  • Gene Name:
  • EIF2S1
  • Gene Alias:
  • EIF-2,EIF-2A,EIF-2alpha,EIF2,EIF2A
  • Gene Description:
  • eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
  • Gene Summary:
  • The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM
  • Other Designations:
  • eIF-2-alpha,eukaryotic translation initiation factor 2, subunit 1 (alpha, 35kD )
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