DOK2 (phospho Y299) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from K-562 cells using DOK2 (phospho Y299) polyclonal antibody (Cat #PAB25860).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using DOK2 (phospho Y299) polyclonal antibody (Cat # PAB25860).
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using DOK2 (phospho Y299) polyclonal antibody (Cat # PAB25860).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of DOK2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y299 of human DOK2.
Sequence
G-E-Yp-A-V
Host
Rabbit
Theoretical MW (kDa)
56
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Concentration
1 mg/mL
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
Immunofluorescence (1:100-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from K-562 cells using DOK2 (phospho Y299) polyclonal antibody (Cat #PAB25860).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using DOK2 (phospho Y299) polyclonal antibody (Cat # PAB25860).Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using DOK2 (phospho Y299) polyclonal antibody (Cat # PAB25860). -
Gene Info — DOK2
Entrez GeneID
9046Protein Accession#
O60496Gene Name
DOK2
Gene Alias
p56DOK, p56dok-2
Gene Description
docking protein 2, 56kDa
Omim ID
604997Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq
Other Designations
docking protein 2
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Interactome
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Disease
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