NKX2-6 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NKX2-6.
Immunogen
A synthetic peptide corresponding to 15 amino acids at internal region of human NKX2-6.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
NKX2-6 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-6 are known to exist; this antibody will detect both isoforms.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of NKX2-6 in mouse spleen tissue with NKX2-6 polyclonal antibody (Cat # PAB25800) at 1 ug/mL in (lane 1) the absence and (lane 2) the presence of blocking peptide.Immunohistochemistry
Immunohistochemical analysis of NKX2-6 in human spleen tissue with NKX2-6 polyclonal antibody (Cat # PAB25800) at 5 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NKX2-6
Entrez GeneID
137814Gene Name
NKX2-6
Gene Alias
CSX2, NKX4-2
Gene Description
NK2 transcription factor related, locus 6 (Drosophila)
Gene Ontology
HyperlinkGene Summary
Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is essential for development of the heart-like dorsal vessel. NKX2-6 is a vertebrate homolog of 'tinman' (Newman and Krieg, 1998 [PubMed 9621430]).[supplied by OMIM
Other Designations
NK2 transcription factor related, locus 6|tinman paralog
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