NKX2-1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NKX2-1.
Immunogen
A synthetic peptide corresponding to 16 amino acids at N-terminus of human NKX2-1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
NKX2-1 antibody is predicted to not cross-react with other NK2 homeobox family members. At least three isoforms of NKX2-1 are known to exist; this antibody will detect all three.
Form
Liquid
Purification
Peptide affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (20 ug/mL)
Immunohistochemistry (2.5 ug/mL)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide).
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of NKX2-1 in rat lung tissue with NKX2-1 polyclonal antibody (Cat # PAB25797) at (lane 1) 1 and (lane 2) 2 ug/mL.Immunohistochemistry
Immunohistochemical analysis of NKX2-1 in human lung tissue with NKX2-1 polyclonal antibody (Cat # PAB25797) at 2.5 ug/mL.Immunofluorescence
Immunofluorescence staining of human lung tissue with NKX2-1 polyclonal antibody (Cat # PAB25797) under 20 ug/mL working concentration.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NKX2-1
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Interactome
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Disease
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