RAPGEF4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RAPGEF4.
Immunogen
A synthetic peptide corresponding to 15 amino acids at N-terminus of human RAPGEF4.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
At least two isoforms of EPAC2 are known to exist; this antibody will detect only the larger isoform. EPAC2 antibody is predicted to not cross-react with EPAC2.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (2.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of RAPGEF4 in rat liver tissue with RAPGEF4 polyclonal antibody (Cat # PAB25776) at 1 ug/mL in (lane 1) the absence and (lane 2) the presence of blocking peptide.Immunohistochemistry
Immunohistochemical analysis of RAPGEF4 in mouse liver tissue with RAPGEF4 polyclonal antibody (Cat # PAB25776) at 2.5 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RAPGEF4
Entrez GeneID
11069Protein Accession#
NP_008954Gene Name
RAPGEF4
Gene Alias
CAMP-GEFII, CGEF2, EPAC2, Nbla00496
Gene Description
Rap guanine nucleotide exchange factor (GEF) 4
Omim ID
606058Gene Ontology
HyperlinkOther Designations
OTTHUMP00000165043|OTTHUMP00000165045|RAP guanine-nucleotide-exchange factor (GEF) 4|cAMP-regulated guanine nucleotide exchange factor II|exchange protein directly activated by cAMP 2|putative protein product of Nbla00496
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Interactome
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