PRICKLE4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRICKLE4.
Immunogen
A synthetic peptide corresponding to 18 amino acids at N-terminus of human PRICKLE4.
Host
Rabbit
Reactivity
Human
Specificity
Two isoforms of PRICKLE4 are known to exist. PRICKLE4 antibody is predicted to not cross-react with other PRICKLE protein family members.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Isotype
IgG
Recommend Usage
Western Blot (0.25 ug/mL)
Immunocytochemistry (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PRICKLE4 in A-549 cell lysate with PRICKLE4 polyclonal antibody (Cat # PAB25747) at 0.25 ug/ml in the (lane 1) absence and (lane 2) presence of blocking peptide.Immunocytochemistry
Immunocytochemical anyalysis of PRICKLE4 in A-549 cell lysate with PRICKLE4 polyclonal antibody (Cat # PAB25747) at 5 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRICKLE4
Entrez GeneID
29964Protein Accession#
NP_037529Gene Name
PRICKLE4
Gene Alias
C6orf49, MGC120398, OBTP, OEBT
Gene Description
prickle homolog 4 (Drosophila)
Omim ID
611389Gene Ontology
HyperlinkGene Summary
C6ORF49 is a member of the LIM domain protein family (Teufel et al., 2005 [PubMed 15702247]).[supplied by OMIM
Other Designations
OTTHUMP00000174832|over-expressed breast tumor protein
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