NANOS2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CEM cell lysate (35 ug/lane) with NANOS2 polyclonal antibody (Cat # PAB2574).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testis tissue reacted with NANOS2 polyclonal antibody (Cat # PAB2574) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NANOS2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NANOS2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CEM cell lysate (35 ug/lane) with NANOS2 polyclonal antibody (Cat # PAB2574).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testis tissue reacted with NANOS2 polyclonal antibody (Cat # PAB2574) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — NANOS2
Entrez GeneID
339345Protein Accession#
NP_001025032;P60321Gene Name
NANOS2
Gene Alias
NOS2
Gene Description
nanos homolog 2 (Drosophila)
Omim ID
608228Gene Ontology
HyperlinkOther Designations
nanos homolog 2
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Publication Reference
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Expression of nitric oxide synthases in keratinocytes after UVB irradiation.
Chang HR, Tsao DA, Wang SR, Yu HS.
Archives of Dermatological Research 2003 Dec; 295(7):293.
Application：WB, Human, Human keratinocytes.
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Conserved role of nanos proteins in germ cell development.
Tsuda M, Sasaoka Y, Kiso M, Abe K, Haraguchi S, Kobayashi S, Saga Y.
Science 2003 Aug; 301(5637):1239.
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Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells.
Jaruzelska J, Kotecki M, Kusz K, Spik A, Firpo M, Reijo Pera RA.
Development Genes and Evolution 2003 Apr; 213(3):120.
Application：IHC-P, Human, Human testis sections.
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Expression of nitric oxide synthases in keratinocytes after UVB irradiation.
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