LECT1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of LECT1 polyclonal antibody (Cat # PAB2573) in NCI-H460 cell line lysates (35 ug/lane).LECT1 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinomareacted with LECT1 polyclonal antibody (Cat # PAB2573), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LECT1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human LECT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of LECT1 polyclonal antibody (Cat # PAB2573) in NCI-H460 cell line lysates (35 ug/lane).LECT1 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinomareacted with LECT1 polyclonal antibody (Cat # PAB2573), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — LECT1
Entrez GeneID
11061Protein Accession#
NP_008946;O75829Gene Name
LECT1
Gene Alias
BRICD3, CHM-I, CHM1
Gene Description
leukocyte cell derived chemotaxin 1
Omim ID
605147Gene Ontology
HyperlinkGene Summary
This gene encodes a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This gene is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
BRICHOS domain containing 3|OTTHUMP00000018466|chondromodulin I
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Interactome
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Disease
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Publication Reference
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Cell-specific epigenetic regulation of ChM-I gene expression: crosstalk between DNA methylation and histone acetylation.
Aoyama T, Okamoto T, Kohno Y, Fukiage K, Otsuka S, Furu M, Ito K, Jin Y, Nagayama S, Nakayama T, Nakamura T, Toguchida J.
Biochemical and Biophysical Research Communications 2008 Jan; 365(1):124.
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Chondromodulin-I maintains cardiac valvular function by preventing angiogenesis.
Yoshioka M, Yuasa S, Matsumura K, Kimura K, Shiomi T, Kimura N, Shukunami C, Okada Y, Mukai M, Shin H, Yozu R, Sata M, Ogawa S, Hiraki Y, Fukuda K.
Nature Medicine 2006 Oct; 12(10):1151.
Application：IHC, IF, Human, Mouse, Heart, VICs, NIH3T3, HCAEC cells, human cardiac valves.
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Methylation in the core-promoter region of the chondromodulin-I gene determines the cell-specific expression by regulating the binding of transcriptional activator Sp3.
Aoyama T, Okamoto T, Nagayama S, Nishijo K, Ishibe T, Yasura K, Nakayama T, Nakamura T, Toguchida J.
The Journal of Biological Chemistry 2004 Jul; 279(27):28789.
Application：WB, Human, ANOS, HOS, HuO, MG63, SaOS2, TAKAO, U2OS cells.
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Cell-specific epigenetic regulation of ChM-I gene expression: crosstalk between DNA methylation and histone acetylation.
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