DPAGT1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DPAGT1.
Immunogen
A synthetic peptide corresponding to 17 amino acids at N-terminus of human DPAGT1.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
At least four isoforms of DPAGT1 are known to exist; this antibody will recognize the two longest isoforms. DPAGT1 antibody is predicted to not cross-react with UHRF1BP1.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (2.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of DPAGT1 in mouse kidney tissue with DPAGT1 polyclonal antibody (Cat # PAB25725) at 1 ug/mL in (lane 1) the absence and (lane 2) the presence of blocking peptide.Immunohistochemistry
Immunohistochemical analysis of DPAGT1 in human kidney tissue with DPAGT1 polyclonal antibody (Cat # PAB25725) at 2.5 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DPAGT1
Entrez GeneID
1798Protein Accession#
NP_001373Gene Name
DPAGT1
Gene Alias
ALG7, CDG-Ij, D11S366, DGPT, DPAGT, DPAGT2, G1PT, GPT, UAGT, UGAT
Gene Description
dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme. [provided by RefSeq
Other Designations
GlcNAc-1-P transferase|N-acetylglucosamine-1-phosphate transferase|UDP-GlcNAc:dolichyl-phosphate N-acetylglucosaminephosphotransferase|UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase|dolichyl-phosphate alpha-N-acetylglucos
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Interactome
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Pathway
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Disease
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