POU3F2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of POU3F2.
Immunogen
A synthetic peptide corresponding to 18 amino acids at C-terminus of human POU3F2.
Host
Rabbit
Theoretical MW (kDa)
49
Reactivity
Human, Mouse, Rat
Specificity
At least two isoforms are known to exist. This antibody will recognize both isoforms. POU3F2 antibody is predicted to not cross-react with other members of the POU domain class 3 family.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Isotype
IgG
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of POU3F2 in NIH/3T3 cell lysate with POU3F2 polyclonal antibody (Cat # PAB25679) at (1) 1 and (2) 2 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — POU3F2
Entrez GeneID
5454Protein Accession#
NP_005595Gene Name
POU3F2
Gene Alias
BRN2, OCT7, OTF7, POUF3
Gene Description
POU class 3 homeobox 2
Omim ID
600494Gene Ontology
HyperlinkGene Summary
POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM
Other Designations
POU domain, class 3, transcription factor 2
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