CENPW polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CENPW.
Immunogen
A synthetic peptide corresponding to 16 amino acids at N-terminus of human CENPW.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
At least three isoforms of CENPW are known to exist; this antibody will detect all three isoforms.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Isotype
IgG
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CENPW in HeLa cell lysate with CENPW polyclonal antibody (Cat # PAB25656) at (1) 0.5 and (2) 1 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CENPW
Entrez GeneID
387103Protein Accession#
NP_001012525Gene Name
CENPW
Gene Alias
CUG2, C6orf173
Gene Description
centromere protein W
Omim ID
611264Gene Ontology
HyperlinkOther Designations
OTTHUMP00000017157|cancer-upregulated gene 2|hypothetical protein LOC387103
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Interactome
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Disease
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Publication Reference
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CENP-W inhibits CDC25A degradation by destabilizing the SCFβ-TrCP-1 complex at G2/M.
Cheon Y, Lee S.
FASEB Journal 2018 Jun; [Epub].
Application:WB, WB-Tr, Human, 293T cells.
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Centromere Protein (CENP)-W Interacts with Heterogeneous Nuclear Ribonucleoprotein (hnRNP) U and May Contribute to Kinetochore-Microtubule Attachment in Mitotic Cells.
Chun Y, Kim R, Lee S.
PloS One 2016 Feb; 11(2):e0149127.
Application:IP, Human, 293T, HeLa-CENP-W cells.
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CENP-W inhibits CDC25A degradation by destabilizing the SCFβ-TrCP-1 complex at G2/M.
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