WIF1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of WIF1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 350-379 at C-terminus of human WIF1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemsitry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of WIF1 (arrow) using WIF1 polyclonal antibody (Cat # PAB2564). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the WIF1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 polyclonal antibody (Cat # PAB2564) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — WIF1
Entrez GeneID
11197Protein Accession#
NP_009122;Q9Y5W5Gene Name
WIF1
Gene Alias
WIF-1
Gene Description
WNT inhibitory factor 1
Omim ID
605186Gene Ontology
HyperlinkGene Summary
WNT proteins are extracellular signaling molecules involved in the control of embryonic development. This gene encodes a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Wnt pathway inhibitors are strongly down-regulated in pituitary tumors.
Elston MS, Gill AJ, Conaglen JV, Clarkson A, Shaw JM, Law AJ, Cook RJ, Little NS, Clifton-Bligh RJ, Robinson BG, McDonald KL.
Endocrinology 2008 Mar; 149(3):1235.
Application:IHC-P, WB,-Tr, Human, Rat, Pituitary tumors, WIF1-transfected GH3 cells, EV-transfected GH3 cells, GH3 cells.
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Epigenetic alteration of the Wnt inhibitory factor-1 promoter occurs early in the carcinogenesis of Barrett's esophagus.
Clement G, Guilleret I, He B, Yagui-Beltran A, Lin YC, You L, Xu Z, Shi Y, Okamoto J, Benhattar J, Jablons D.
Cancer Science 2008 Jan; 99(1):46.
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The tumor suppressor Wnt inhibitory factor 1 is frequently methylated in nasopharyngeal and esophageal carcinomas.
Chan SL, Cui Y, van Hasselt A, Li H, Srivastava G, Jin H, Ng KM, Wang Y, Lee KY, Tsao GS, Zhong S, Robertson KD, Rha SY, Chan AT, Tao Q.
Laboratory Investigation 2007 Jul; 87(7):644.
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Wnt pathway inhibitors are strongly down-regulated in pituitary tumors.
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