PHPT1 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of PHPT1 (arrow) using rabbit PHPT1 polyclonal antibody (Cat # PAB2560). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHPT1 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PHPT1 polyclonal antibody (Cat # PAB2560) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PHPT1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PHPT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of PHPT1 (arrow) using rabbit PHPT1 polyclonal antibody (Cat # PAB2560). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHPT1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PHPT1 polyclonal antibody (Cat # PAB2560) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — PHPT1
Entrez GeneID
29085GeneBank Accession#
NM_014172; Q9NRX4Gene Name
PHPT1
Gene Alias
CGI-202, DKFZp564M173, HSPC141, PHP14, bA216L13.10
Gene Description
phosphohistidine phosphatase 1
Omim ID
610167Gene Ontology
HyperlinkGene Summary
PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I (Ek et al., 2002 [PubMed 12383260]).[supplied by OMIM
Other Designations
1700008C22Rik|OTTHUMP00000022619|phosphohistidine phosphatase 14kDa|sex-regulated protein janus-a
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Interactome
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Pathway
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Publication Reference
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A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration.
Lim J, Hao T, Shaw C, Patel AJ, Szabo G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabasi AL, Vidal M, Zoghbi HY.
Cell 2006 May; 125(4):801.
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The LIFEdb database in 2006.
Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S.
Nucleic Acids Research 2006 Jan; 34:D415.
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Mutational study of human phosphohistidine phosphatase: effect on enzymatic activity.
Ma R, Kanders E, Sundh UB, Geng M, Ek P, Zetterqvist O, Li JP.
Biochemical and Biophysical Research Communications 2005 Nov; 337(3):887.
Application：WB-Re, Recombinant protein.
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A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration.
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