Jph3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of Jph3 polyclonal antibody (Cat # PAB2555) in mouse brain tissue lysates (35 ug/lane). Jph3 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with Jph3 polyclonal antibody (Cat # PAB2555) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Jph3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of mouse Jph3.
Host
Rabbit
Reactivity
Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Jph3 polyclonal antibody (Cat # PAB2555) in mouse brain tissue lysates (35 ug/lane). Jph3 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with Jph3 polyclonal antibody (Cat # PAB2555) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — Jph3
Entrez GeneID
57340Protein Accession#
NP_065630;Q9ET77Gene Name
Jph3
Gene Alias
JP-3, Jp3, MGC124338, MGC124339, MGC124340
Gene Description
junctophilin 3
Gene Ontology
HyperlinkOther Designations
junctophilin type 3
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Publication Reference
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Junctophilin-mediated channel crosstalk essential for cerebellar synaptic plasticity.
Kakizawa S, Kishimoto Y, Hashimoto K, Miyazaki T, Furutani K, Shimizu H, Fukaya M, Nishi M, Sakagami H, Ikeda A, Kondo H, Kano M, Watanabe M, Iino M, Takeshima H.
The EMBO Journal 2007 Mar; 26(7):1924.
Application：WB-Ti, Mouse, Mouse cerebellum.
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Functional uncoupling between Ca2+ release and afterhyperpolarization in mutant hippocampal neurons lacking junctophilins.
Moriguchi S, Nishi M, Komazaki S, Sakagami H, Miyazaki T, Masumiya H, Saito SY, Watanabe M, Kondo H, Yawo H, Fukunaga K, Takeshima H.
PNAS 2006 Jun; 103(28):10811.
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Coexpression of junctophilin type 3 and type 4 in brain.
Nishi M, Sakagami H, Komazaki S, Kondo H, Takeshima H.
Brain Research. Molecular Brain Research 2003 Oct; 118(1-2):102.
Application：WB-Ti, Mouse, Mouse brain, liver, kidney.
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Junctophilin-mediated channel crosstalk essential for cerebellar synaptic plasticity.
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