Arhgef9 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Arhgef9.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 281-311 at internal region of rat Arhgef9.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Arhgef9 (arrow) using rabbit Arhgef9 polyclonal antibody (Cat # PAB2551). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Arhgef9 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with Arhgef9 polyclonal antibody (Cat # PAB2551) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — Arhgef9
Entrez GeneID
66013Protein Accession#
NP_076447;Q9QX73Gene Name
Arhgef9
Gene Alias
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Gene Description
Cdc42 guanine nucleotide exchange factor (GEF) 9
Gene Ontology
HyperlinkOther Designations
collybistin I
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Publication Reference
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The crystal structure of Cdc42 in complex with collybistin II, a gephyrin-interacting guanine nucleotide exchange factor.
Xiang S, Kim EY, Connelly JJ, Nassar N, Kirsch J, Winking J, Schwarz G, Schindelin H.
Journal of Molecular Biology 2006 May; 359(1):35.
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The GDP-GTP exchange factor collybistin: an essential determinant of neuronal gephyrin clustering.
Harvey K, Duguid IC, Alldred MJ, Beatty SE, Ward H, Keep NH, Lingenfelter SE, Pearce BR, Lundgren J, Owen MJ, Smart TG, Luscher B, Rees MI, Harvey RJ.
Journal of Neuroscience 2004 Jun; 24(25):5816.
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Identification of a gephyrin-binding motif in the GDP/GTP exchange factor collybistin.
Grosskreutz Y, Hermann A, Kins S, Fuhrmann JC, Betz H, Kneussel M.
Biological Chemistry 2001 Oct; 382(10):1455.
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The crystal structure of Cdc42 in complex with collybistin II, a gephyrin-interacting guanine nucleotide exchange factor.
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