Flag tag polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Flag tag.
Immunogen
A synthetic peptide corresponding to Flag tag.
Sequence
DYKDDDDK
Host
Rabbit
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of extract from 293 cells transfected with Flag-tagged Bad using Flag tag polyclonal antibody (Cat # PAB25505). -
Publication Reference
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The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis.
Jing Zhang, Zechen Bai, Min Ouyang, Xiumei Xu, Haibo Xiong, Qiang Wang, Bernhard Grimm, Jean-David Rochaix, Lixin Zhang.
The EMBO Journal 2021 Jul; 40(13):e106742.
Application:IP, WB-Tr, Plant, Plant protoplasts.
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Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway.
Zhang L, Xu C, Hu W, Wu P, Qin C, Zhang J.
International Journal of Molecular Medicine 2018 Mar; 41(3):1293.
Application:WB, Rat, NRK‑52E cells.
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The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis.
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