RIF1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RIF1.
Immunogen
A synthetic peptide corresponding to C-terminus of human RIF1.
Sequence
CRNLQSRWRSPSHEN
Host
Goat
Theoretical MW (kDa)
270
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
RIF1 polyclonal antibody (Cat # PAB25455) (1 ug/mL) staining of HeLa and Mouse D3 (ES) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Data obtained from Hisao Masai, Tokyo Metropolitan Institure of Medical Science, Japan.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RIF1
Entrez GeneID
55183Protein Accession#
NP_060621.3;NP_001171134.1Gene Name
RIF1
Gene Alias
DKFZp434D1026, DKFZp781N1478, FLJ12870
Gene Description
RAP1 interacting factor homolog (yeast)
Omim ID
608952Gene Ontology
HyperlinkOther Designations
OTTHUMP00000162712|RAP1 interacting factor 1|Rap1 interacting factor 1
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Interactome
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