SCYE1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
SCYE1 polyclonal antibody (Cat # PAB25441) (0.3 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SCYE1.
Immunogen
A synthetic peptide corresponding to N-terminus of human SCYE1.
Sequence
NNDAVLKRLEQKC
Host
Goat
Theoretical MW (kDa)
37
Reactivity
Human, Mouse, Rat
Specificity
This antibody is expected to recognize both reported isoforms (NP_004748.2; NP_001135888.1). Reported variants represent identical protein: NP_004748.2, NP_001135887.1
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SCYE1 polyclonal antibody (Cat # PAB25441) (0.3 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SCYE1
Entrez GeneID
9255Protein Accession#
NP_004748.2;NP_001135888.1Gene Name
SCYE1
Gene Alias
AIMP1, EMAP2, EMAPII, p43
Gene Description
small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
Omim ID
603605Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq
Other Designations
ARS-interacting multifunctional protein 1|OTTHUMP00000161878|endothelial monocyte-activating polypeptide 2|endothelial-monocyte activating polypeptide II|multisynthetase complex auxiliary component p43|small inducible cytokine subfamily E, member 1
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Interactome
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Disease
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