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Last updated: 2016/12/4
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KDR (phospho Y1214) polyclonal antibody

  • Catalog # : PAB25304
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of KDR.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding Y1214 of human KDR.
  • Sequence:
  • F-H-Yp-D-N
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 230
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Affinity chromatography
  • Concentration:
  • 1 mg/mL
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1:1000)
    Immunofluorescence (1:100-1:200)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extracts from SK-OV-3 cells using KDR (phospho Y1214) polyclonal antibody (Cat # PAB25304).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using KDR (phospho Y1214) polyclonal antibody (Cat # PAB25304).
  • Immunofluorescence
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Gene Information
  • Entrez GeneID:
  • 3791
  • Gene Name:
  • KDR
  • Gene Alias:
  • CD309,FLK1,VEGFR,VEGFR2
  • Gene Description:
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Gene Summary:
  • Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq
  • Other Designations:
  • soluble VEGFR2,vascular endothelial growth factor receptor 2
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