MEF2A (phospho T319) polyclonal antibody
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More Files
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Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using MEF2A (phospho T319) polyclonal antibody (Cat # PAB25261).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MEF2A.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T319 of human MEF2A.
Sequence
V-T-Tp-P-S
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
MEF2A (Phospho-Thr319) antibody detects endogenous levels of MEF2A only when phosphorylated at Thr319.
Form
Liquid
Purification
Affinity chromatography
Concentration
1 mg/mL
Recommend Usage
Immunofluorescence (1:100-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using MEF2A (phospho T319) polyclonal antibody (Cat # PAB25261). -
Gene Info — MEF2A
Entrez GeneID
4205Protein Accession#
Q02078Gene Name
MEF2A
Gene Alias
ADCAD1, RSRFC4, RSRFC9
Gene Description
myocyte enhancer factor 2A
Gene Ontology
HyperlinkGene Summary
The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain.[supplied by OMIM
Other Designations
MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A)
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Interactome
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Disease
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