CYB5R3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with CYB5R3 polyclonal antibody (Cat # PAB25136) at 1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human placenta tissue using CYB5R3 polyclonal antibody (Cat # PAB25136).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CYB5R3.
Immunogen
A synthetic peptide corresponding to CYB5R3.
Host
Rabbit
Theoretical MW (kDa)
~34.0
Reactivity
Human
Specificity
CYB5R3 polyclonal antibody detects endogenous levels of CYB5R3 protein.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with CYB5R3 polyclonal antibody (Cat # PAB25136) at 1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human placenta tissue using CYB5R3 polyclonal antibody (Cat # PAB25136). -
Gene Info — CYB5R3
Entrez GeneID
1727Gene Name
CYB5R3
Gene Alias
B5R, DIA1
Gene Description
cytochrome b5 reductase 3
Omim ID
250800Gene Ontology
HyperlinkGene Summary
This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. These two forms are resulted from alternative splicing of the gene. Mutations in this gene cause methemoglobinemias. [provided by RefSeq
Other Designations
NADH-cytochrome b5 reductase|OTTHUMP00000028761|cytochrome b5 reductase|diaphorase (NADH) (cytochrome b-5 reductase)
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Interactome
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Disease
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