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Last updated: 2016/12/4
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ACSL4 polyclonal antibody

  • Catalog # : PAB2504
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ACSL4.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 244 -274 at internal region of human ACSL4.
  • Sequence:
  • IHSMQSVEELGSNPENLGIPPSRPTPSDMAI
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of HepG2 whole cell lysate with ACSL4 polyclonal antibody (Cat # PAB2504) followed by HRP conjugated goat anti-rabbit IgG (H+L) as second antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the ACSL4 polyclonal antibody (Cat # PAB2504) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2182
  • Gene Name:
  • ACSL4
  • Gene Alias:
  • ACS4,FACL4,LACS4,MRX63,MRX68
  • Gene Description:
  • acyl-CoA synthetase long-chain family member 4
  • Gene Summary:
  • The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000023846,acyl-CoA synthetase 4,fatty-acid-Coenzyme A ligase, long-chain 4,lignoceroyl-CoA synthase,long-chain fatty-acid-Coenzyme A ligase 4
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