TRIM25 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TRIM25.
Immunogen
A synthetic peptide corresponding to TRIM25.
Host
Rabbit
Theoretical MW (kDa)
71
Reactivity
Human
Specificity
TRIM25 polyclonal antibody detects endogenous levels of TRIM25 protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
1 mg/mL
Purity
> 95% by SDS-PAGE
Recommend Usage
Western Blot (1:500-1000)
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In 1x PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K-562 cell lysate with TRIM25 polyclonal antibody (Cat # PAB24887).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Gene Info — TRIM25
Entrez GeneID
7706Gene Name
TRIM25
Gene Alias
EFP, RNF147, Z147, ZNF147
Gene Description
tripartite motif-containing 25
Omim ID
600453Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq
Other Designations
estrogen-responsive finger protein|tripartite motif protein TRIM25|zinc finger protein 147 (estrogen-responsive finger protein)|zinc finger protein-147
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Interactome
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Disease
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