NANOS1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NANOS1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NANOS1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunofluorescence (1:10-50)
Immunohistochemistry (1:10-50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NANOS1 polyclonal antibody (Cat # PAB2486) in T-47D cell line lysates (35 ug/lane). NANOS1 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 polyclonal antibody (Cat # PAB2486) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.Immunofluorescence
Immunofluorescence analysis of NANOS1 polyclonal antibody (Cat # PAB2486) in HeLa cells. Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.Flow Cytometry
Flow cytometric analysis of HeLa cells using NANOS1 polyclonal antibody (Cat # PAB2486)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — NANOS1
Entrez GeneID
340719Protein Accession#
NP_955631;Q8WY41Gene Name
NANOS1
Gene Alias
NOS1
Gene Description
nanos homolog 1 (Drosophila)
Omim ID
608226Gene Ontology
HyperlinkOther Designations
OTTHUMP00000046853|nanos homolog 1
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Publication Reference
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E-cadherin regulates human Nanos1, which interacts with p120ctn and induces tumor cell migration and invasion.
Strumane K, Bonnomet A, Stove C, Vandenbroucke R, Nawrocki-Raby B, Bruyneel E, Mareel M, Birembaut P, Berx G, van Roy F.
Cancer Research 2006 Oct; 66(20):10007.
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Time-lapse analysis reveals different modes of primordial germ cell migration in the medaka Oryzias latipes.
Kurokawa H, Aoki Y, Nakamura S, Ebe Y, Kobayashi D, Tanaka M.
Development, Growth & Differentiation 2006 Apr; 48(3):209.
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E-cadherin regulates human Nanos1, which interacts with p120ctn and induces tumor cell migration and invasion.
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