Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 polyclonal antibody (Cat # PAB2486) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Immunofluorescence analysis of NANOS1 polyclonal antibody (Cat # PAB2486) in HeLa cells. Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Flow cytometric analysis of HeLa cells using NANOS1 polyclonal antibody (Cat # PAB2486)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.