GNL3 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GNL3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 465-495 at C-terminus of humanGNL3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HL-60 cell lysate with GNL3 polyclonal antibody (Cat # PAB2481).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with GNL3 polyclonal antibody (Cat # PAB2481) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — GNL3
Entrez GeneID
26354Protein Accession#
NP_055181;Q9BVP2Gene Name
GNL3
Gene Alias
C77032, E2IG3, MGC800, NS
Gene Description
guanine nucleotide binding protein-like 3 (nucleolar)
Omim ID
608011Gene Ontology
HyperlinkOther Designations
estradiol-induced nucleotide binding protein|guanine nucleotide binding protein-like 3|nucleostemin
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Interactomes
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Diseases
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Publication Reference
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Depletion of the nucleolar protein nucleostemin causes G1 cell cycle arrest via the p53 pathway.
Ma H, Pederson T.
Molecular Biology of the Cell 2007 Jul; 18(7):2630.
Application:IF, WB, Human, HeLa, U2OS cells.
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Depletion of the nucleolar protein nucleostemin causes G1 cell cycle arrest via the p53 pathway.
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