DDX4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of DDX4 polyclonal antibody (Cat # PAB2479) in K-562 cell line lysates (35 ug/lane). DDX4 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 polyclonal antibody (Cat # PAB2479) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DDX4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DDX4.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of DDX4 polyclonal antibody (Cat # PAB2479) in K-562 cell line lysates (35 ug/lane). DDX4 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 polyclonal antibody (Cat # PAB2479) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — DDX4
Entrez GeneID
54514Protein Accession#
NP_077726;Q9NQI0Gene Name
DDX4
Gene Alias
MGC111074, VASA
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Omim ID
605281Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. [provided by RefSeq
Other Designations
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4|OTTHUMP00000122546
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Interactome
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Publication Reference
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Differential expression of VASA gene in ejaculated spermatozoa from normozoospermic men and patients with oligozoospermia.
Guo X, Gui YT, Tang AF, Lu LH, Gao X, Cai ZM.
Asian Journal of Andrology 2007 May; 9(3):339.
Application：IF, WB, Human, Human sperm.
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VASA is a specific marker for both normal and malignant human germ cells.
Zeeman AM, Stoop H, Boter M, Gillis AJ, Castrillon DH, Oosterhuis JW, Looijenga LH.
Laboratory Investigation 2002 Feb; 82(2):159.
Application：IHC-P, Human, Testicular tissues.
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The human VASA gene is specifically expressed in the germ cell lineage.
Castrillon DH, Quade BJ, Wang TY, Quigley C, Crum CP.
PNAS 2000 Aug; 97(17):9585.
Application：IHC-P, Human, Testis, Ovary.
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Differential expression of VASA gene in ejaculated spermatozoa from normozoospermic men and patients with oligozoospermia.
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