Western blot analysis of CDH8 (arrow) using rabbit CDH8 polyclonal antibody (Cat # PAB2476). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH8 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human breast carcinomareacted with CDH8 polyclonal antibody (Cat # PAB2476), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed in brain and is putatively involved in synaptic adhesion, axon outgrowth and guidance. [provided by RefSeq