Western blot analysis of NES polyclonal antibody (Cat # PAB24692) in K-562 cell line lysates (35 ug/lane).
Flow cytometry analysis of K-562 cells (bottom histogram) compared to a negative control cell (top histogram). Using NES polyclonal antibody (Cat # PAB24692) as primary antibody. FITC-conjugated goat-anti-rabbit secondary antibody were used for the analysis. (35 ug/lane).
Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM