RRAD polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
RRAD polyclonal antibody (Cat # PAB24687) (0.5 ug/ml) staining of wt and KO lysates of mouse heart (100µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
RRAD polyclonal antibody (Cat # PAB24687) (0.5 ug/mL) staining of HEK293 lysates overexpressing several HA-tagged mouse GTPases, including Rrad (10 ug protein in RIPA buffer) and compared with an HA-specific antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RRAD.
Immunogen
A synthetic peptide corresponding to amino acids 36-48 at internal region of human RRAD.
Sequence
CHRRSMPVDERDLQ
Host
Goat
Theoretical MW (kDa)
37
Reactivity
Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5 % BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
RRAD polyclonal antibody (Cat # PAB24687) (0.5 ug/ml) staining of wt and KO lysates of mouse heart (100µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
RRAD polyclonal antibody (Cat # PAB24687) (0.5 ug/mL) staining of HEK293 lysates overexpressing several HA-tagged mouse GTPases, including Rrad (10 ug protein in RIPA buffer) and compared with an HA-specific antibody.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RRAD
Entrez GeneID
6236Protein Accession#
NP_004156.1Gene Name
RRAD
Gene Alias
RAD, RAD1, REM3
Gene Description
Ras-related associated with diabetes
Omim ID
179503Gene Ontology
HyperlinkOther Designations
RAS (RAD and GEM) like GTP binding 3
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