TXNL1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The TXNL1 polyclonal antibody (Cat # PAB2464) is used in Western blot to detect TXNL1 in HL-60 cell lysate.
Immunohistochemistry (Frozen sections)
Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer.
TXNL1 polyclonal antibody (Cat # PAB2464) gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining.
The results correspond precisely to TXNL1 mRNA expression shown with in situ hybridization (JimAcnez A et al. FEBS Lett. 2006, 580(3):960-7) and could be abolished with peptide used for immunization (Blocking peptide, Cat # P0347).
The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion. Staining was done in frozen sections with Vector Elite kit and nickel intensified DAB as a chromogen.
Data courtesy of Prof. Markku Pelto-Huikko, Department of Developmental Biology, Medical School,Tampere University, Finland.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TXNL1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human TXNL1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The TXNL1 polyclonal antibody (Cat # PAB2464) is used in Western blot to detect TXNL1 in HL-60 cell lysate.Immunohistochemistry (Frozen sections)
Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer.
TXNL1 polyclonal antibody (Cat # PAB2464) gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining.
The results correspond precisely to TXNL1 mRNA expression shown with in situ hybridization (JimAcnez A et al. FEBS Lett. 2006, 580(3):960-7) and could be abolished with peptide used for immunization (Blocking peptide, Cat # P0347).
The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion. Staining was done in frozen sections with Vector Elite kit and nickel intensified DAB as a chromogen.
Data courtesy of Prof. Markku Pelto-Huikko, Department of Developmental Biology, Medical School,Tampere University, Finland. -
Gene Info — TXNL1
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Interactome
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Disease
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Publication Reference
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Molecular cloning and expression of a cDNA encoding a human thioredoxin-like protein.
Miranda-Vizuete A, Gustafsson JA, Spyrou G.
Biochemical and Biophysical Research Communications 1998 Feb; 243(1):284.
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Molecular cloning and expression of a cDNA encoding a human thioredoxin-like protein.
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