The TXNL1 polyclonal antibody (Cat # PAB2464) is used in Western blot to detect TXNL1 in HL-60 cell lysate.
Immunohistochemistry (Frozen sections)
Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer. TXNL1 polyclonal antibody (Cat # PAB2464) gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining. The results correspond precisely to TXNL1 mRNA expression shown with in situ hybridization (JimAcnez A et al. FEBS Lett. 2006, 580(3):960-7) and could be abolished with peptide used for immunization (Blocking peptide, Cat # P0347). The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion. Staining was done in frozen sections with Vector Elite kit and nickel intensified DAB as a chromogen. Data courtesy of Prof. Markku Pelto-Huikko, Department of Developmental Biology, Medical School,Tampere University, Finland.