Cdkn2a polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of Cdkn2a.
Immunogen
A synthetic peptide corresponding to internal region of mouse Cdkn2a.
Sequence
C-RRGPHRNPGPGDDD
Host
Goat
Theoretical MW (kDa)
18
Reactivity
Mouse
Specificity
This antibody is expected to recognize isoform p19ARF (NP_034007.1) only.
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Cdkn2a polyclonal antibody (Cat # PAB24630) (0.3 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Cdkn2a
Entrez GeneID
12578Protein Accession#
NP_034007.1Gene Name
Cdkn2a
Gene Alias
ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19<ARF>, p19ARF
Gene Description
cyclin-dependent kinase inhibitor 2A
Gene Ontology
HyperlinkGene Summary
inhibits CDK4)|cyclin-dependent kinase inhibitor protein|mitochondrial smARF
Other Designations
OTTMUSP00000008143|cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4)|cyclin-dependent kinase inhibitor protein|mitochondrial smARF
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