App polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of App.
Immunogen
A synthetic peptide corresponding to N-terminus of mouse App.
Sequence
DAEFGHDSGFEVRHQKC
Host
Goat
Theoretical MW (kDa)
100
Reactivity
Mouse, Rat
Specificity
This antibody is expected to recognize all reported isoforms of mAPP (NP_001185752.1; NP_031497.2; NP_001185753.1; NP_001185754.1; NP_001185755.1) and to recognize all lengths of the putative mouse Amyloid beta peptide.
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:64000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
App polyclonal antibody (Cat # PAB24627) (0.03 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — App
Entrez GeneID
11820Protein Accession#
NP_001185752.1;NP_031497.2;NP_001185753.1;NP_001185754.1;NP_001185755.1Gene Name
App
Gene Alias
AL024401, Abeta, Adap, Cvap, E030013M08Rik, appican, betaAPP
Gene Description
amyloid beta (A4) precursor protein
Gene Ontology
HyperlinkOther Designations
protease nexin II
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