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UBE2C polyclonal antibody

  • Catalog # : PAB24608
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of UBE2C.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human UBE2C.
  • Sequence:
  • C-SGDKGISAFPESDN
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 19
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize isoforms 1 (NP_008950.1), 2 ( NP_861515.1) and 4 (NP_861517.1). Reported variants NP_861517.1 and NP_861518.1represent identical protein.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Recommend Usage:
  • ELISA (1:8000)
    Western Blot (1-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • UBE2C polyclonal antibody (Cat # PAB24608) (1 ug/mL) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_008950.1;NP_861515.1;NP_861517.1
  • Gene Name:
  • UBE2C
  • Gene Alias:
  • UBCH10,dJ447F3.2
  • Gene Description:
  • ubiquitin-conjugating enzyme E2C
  • Gene Summary:
  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000031653,OTTHUMP00000031655,cyclin-selective ubiquitin carrier protein,mitotic-specific ubiquitin-conjugating enzyme,ubiquitin carrier protein E2-C,ubiquitin-protein ligase C
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