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Last updated: 2016/11/27

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TBP (isoform1) polyclonal antibody

  • Catalog # : PAB24603
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of TBP (isoform1).
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus of human TBP (isoform1).
  • Sequence:
  • DQNNSLPPYAQC
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 40
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize reported isoform 1(NP_003185.1) only.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:64000)
    Western Blot (0.3-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • TBP (isoform1) polyclonal antibody (Cat # PAB24603) (0.3 ug/mL) staining of HeLa nuclear (1) and cytosolic (2) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6908
  • Gene Name:
  • TBP
  • Gene Alias:
  • GTF2D,GTF2D1,MGC117320,MGC126054,MGC126055,SCA17,TFIID
  • Gene Description:
  • TATA box binding protein
  • Gene Summary:
  • Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000017703
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