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Last updated: 2016/11/27

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MYLK polyclonal antibody

  • Catalog # : PAB24595
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of MYLK.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human MYLK.
  • Sequence:
  • C-KDTKNMEAKKLSKD
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 150
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognise all reported isoforms (NP_444253.3; NP_444254.3 ; NP_444255.3 and NP_444256.3).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Recommend Usage:
  • ELISA (1:8000)
    Western Blot (0.01-0.03 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • MYLK polyclonal antibody (Cat # PAB24595) (0.03 ug/mL) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4638
  • Protein Accession#:
  • NP_444253.3;NP_444254.3;NP_444255.3;NP_444256.3
  • Gene Name:
  • MYLK
  • Gene Alias:
  • DKFZp686I10125,FLJ12216,KRP,MLCK,MLCK1,MLCK108,MLCK210,MSTP083,MYLK1,smMLCK
  • Gene Description:
  • myosin light chain kinase
  • Gene Summary:
  • This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000180642,OTTHUMP00000180643,myosin, light polypeptide kinase,smooth muscle myosin light chain kinase
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