MYLK polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
MYLK polyclonal antibody (Cat # PAB24595) (0.03 ug/mL) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MYLK.
Immunogen
A synthetic peptide corresponding to internal region of human MYLK.
Sequence
C-KDTKNMEAKKLSKD
Host
Goat
Theoretical MW (kDa)
150
Reactivity
Human
Specificity
This antibody is expected to recognise all reported isoforms (NP_444253.3; NP_444254.3 ; NP_444255.3 and NP_444256.3).
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:8000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MYLK polyclonal antibody (Cat # PAB24595) (0.03 ug/mL) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MYLK
Entrez GeneID
4638Protein Accession#
NP_444253.3;NP_444254.3;NP_444255.3;NP_444256.3Gene Name
MYLK
Gene Alias
DKFZp686I10125, FLJ12216, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK1, smMLCK
Gene Description
myosin light chain kinase
Omim ID
600922Gene Ontology
HyperlinkGene Summary
This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq
Other Designations
OTTHUMP00000180642|OTTHUMP00000180643|myosin, light polypeptide kinase|smooth muscle myosin light chain kinase
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Interactome
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Pathway
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Disease
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