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Last updated: 2017/12/17

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ATP2B1 polyclonal antibody

  • Catalog # : PAB24566
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of ATP2B1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 312-327 at internal region of human ATP2B1.
  • Sequence:
  • C-KQDGAIENRNKAKAQD
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 150
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_001001323.1; NP_001673.2).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Recommend Usage:
  • ELISA (1:64000)
    Western Blot (0.03-0.1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • ATP2B1 polyclonal antibody (Cat # PAB24566) (0.03 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 490
  • Protein Accession#:
  • NP_001001323.1;NP_001673.2
  • Gene Name:
  • ATP2B1
  • Gene Alias:
  • PMCA1,PMCA1kb
  • Gene Description:
  • ATPase, Ca++ transporting, plasma membrane 1
  • Gene Summary:
  • The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • plasma membrane calcium ATPase 1,plasma membrane calcium pump,plasma membrane calcium-ATPase
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