LYZ polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
LYZ polyclonal antibody (Cat # PAB24550) was used to detect purified Lysozyme under reduced (left) and reduced (Lane 1) and non-reduced (Lane 2) conditions (right).
Blot shown on right shows detection of 0.5 ug reduced and nonreduced Lysozyme using LYZ polyclonal antibody (Cat # PAB24550) (1:1000) with Dylight 549 conjugated secondary antibody (1:1000 1.5 hr RT).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length native LYZ.
Immunogen
Native protein purified from hen egg white.
Host
Rabbit
Reactivity
Chicken
Specificity
A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100% sequence homology. Lysozyme from quail and pheasant are also reported to be 100% identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined.
Form
Liquid
Purification
Salt fractionation and ion exchange chromatography
Recommend Usage
ELISA (1:25000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:500-1:2000)
Immunoprecipitation (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
LYZ polyclonal antibody (Cat # PAB24550) was used to detect purified Lysozyme under reduced (left) and reduced (Lane 1) and non-reduced (Lane 2) conditions (right).
Blot shown on right shows detection of 0.5 ug reduced and nonreduced Lysozyme using LYZ polyclonal antibody (Cat # PAB24550) (1:1000) with Dylight 549 conjugated secondary antibody (1:1000 1.5 hr RT).Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — LYZ
Entrez GeneID
396218Protein Accession#
NP_001001470.1Gene Name
LYZ
Gene Alias
LYZC
Gene Description
lysozyme (renal amyloidosis)
Gene Ontology
HyperlinkOther Designations
dystrophin|egg white lysozyme|lysozyme|lysozyme C
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