Rabbit polyclonal antibody raised against full length native LYZ.
Native protein purified from hen egg white.
A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100% sequence homology. Lysozyme from quail and pheasant are also reported to be 100% identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined.
Salt fractionation and ion exchange chromatography
ELISA (1:25000) Western Blot (1:500-1:2000) Immunohistochemistry (1:500-1:2000) Immunoprecipitation (1:100) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
LYZ polyclonal antibody (Cat # PAB24550) was used to detect purified Lysozyme under reduced (left) and reduced (Lane 1) and non-reduced (Lane 2) conditions (right). Blot shown on right shows detection of 0.5 ug reduced and nonreduced Lysozyme using LYZ polyclonal antibody (Cat # PAB24550) (1:1000) with Dylight 549 conjugated secondary antibody (1:1000 1.5 hr RT).