ANAPC13 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with ANAPC13 polyclonal antibody (Cat # PAB24470).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human stomach with ANAPC13 polyclonal antibody (Cat # PAB24470) shows strong cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant ANAPC13.
Immunogen
Recombinant protein corresponding to amino acids of human ANAPC13.
Sequence
MDSEVQRDGRILDLIDDAWREDKLPYEDVAIPLNELPEPEQDNGGTTESVKEQEMKWTDLALQYLHENVP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with ANAPC13 polyclonal antibody (Cat # PAB24470).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human stomach with ANAPC13 polyclonal antibody (Cat # PAB24470) shows strong cytoplasmic positivity in glandular cells at 1:50-1:200 dilution. -
Gene Info — ANAPC13
Entrez GeneID
25847Protein Accession#
Q9BS18Gene Name
ANAPC13
Gene Alias
APC13, DKFZp566D193, SWM1
Gene Description
anaphase promoting complex subunit 13
Gene Ontology
HyperlinkGene Summary
This gene encodes a component of the anaphase promoting complex, a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. The encoded protein is evolutionarily conserved and is required for the integrity and ubiquitin ligase activity of the anaphase promoting complex. Pseudogenes and splice variants have been found for this gene; however, the biological validity of some of the splice variants has not been determined. [provided by RefSeq
Other Designations
cyclosome subunit 13|homolog of yeast Swm1
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Interactome
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Pathway
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Disease
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