HRK polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse kidney tissue lysate (35 ug/lane) with HRK polyclonal antibody (Cat # PAB2444).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the HRK polyclonal antibody (Cat # PAB2444) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HRK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 30-50 of human HRK.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse kidney tissue lysate (35 ug/lane) with HRK polyclonal antibody (Cat # PAB2444).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the HRK polyclonal antibody (Cat # PAB2444) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — HRK
Entrez GeneID
8739Protein Accession#
NP_003797Gene Name
HRK
Gene Alias
DP5, HARAKIRI
Gene Description
harakiri, BCL2 interacting protein (contains only BH3 domain)
Omim ID
603447Gene Ontology
HyperlinkGene Summary
Activator of apoptosis Hrk regulates apoptosis through interaction with death-repressor proteins Bcl-2 and Bcl-X(L). The HRK protein lacks significant homology to other BCL2 family members except for an 8-amino acid region that was similar to the BCL2 homology domain-3 (BH3) motif of BIK. HRK interacts with BCL2 and BCLXL via the BH3 domain, but not with the death-promoting BCL2-related proteins BAX, BAK, or BCLXS. HRK localizes to membranes of intracellular organelles in a pattern similar to that previously reported for BCL2 and BCLXL. [provided by RefSeq
Other Designations
BCL2-interacting protein|activator of apoptosis Hrk|death protein 5|harakiri
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Disease
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Publication Reference
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Up-regulation of Hrk, a regulator of cell death, in retinal ganglion cells of axotomized rat retina.
Wakabayashi T, Kosaka J, Hommura S.
Neuroscience Letters 2002 Jan; 318(2):77.
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harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-X(L).
Inohara N, Ding L, Chen S, Nunez G.
The EMBO Journal 1997 Apr; 16(7):1686.
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Up-regulation of Hrk, a regulator of cell death, in retinal ganglion cells of axotomized rat retina.
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