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Last updated: 2016/12/4
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BCL2 polyclonal antibody

  • Catalog # : PAB2440
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of BCL2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to the BH3 domain of human BCL2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • BH3 Domain Specific
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:10-50)
    Immunoprecipitation (1:100-500)
    Flow cytometry (1:100-500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Analysis of BCL2 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using BCL2 polyclonal antibody (Cat # PAB2440) for immunoprecipitation (IP) and a different BCL2 antibody for western blot. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.
  • Immunohistochemistry
  • Immunoprecipitation
  • Flow Cytometry
  • Flow Cytometry
  • Analysis of BCL2 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) using BCL2 polyclonal antibody (Cat # PAB2440) in flow cytometry. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 596
  • Protein Accession#:
  • NP_000624;P10415
  • Gene Name:
  • BCL2
  • Gene Alias:
  • Bcl-2
  • Gene Description:
  • B-cell CLL/lymphoma 2
  • Gene Summary:
  • This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq
  • Other Designations:
  • B-cell lymphoma protein 2,OTTHUMP00000163680
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