NOL8 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant NOL8.
Immunogen
Recombinant protein corresponding to amino acids of human NOL8.
Sequence
TDFSLSISNSSDVSAKDKHAEDNEKRLAALEARQKAKEVQKKLVHNALANLDGHPEDKPTHIIFGSDSECETEETSTQEQSHPGEEWVKESMGKTSGKLFDSSDDDESDSEDDSNRFKIKPQFEGRAGQKLMDLQSHFGTDDRFRMDSR
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NOL8 polyclonal antibody (Cat # PAB24335) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebellum with NOL8 polyclonal antibody (Cat # PAB24335) shows strong cytoplasmic and nuclear positivity in purkinje cells at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with NOL8 polyclonal antibody (Cat # PAB24335) at 1-4 ug/mL dilution shows positivity in nucleus and nucleoli. -
Gene Info — NOL8
Entrez GeneID
55035Protein Accession#
Q76FK4Gene Name
NOL8
Gene Alias
C9orf34, DKFZp686P12242, FLJ20736, Nop132, bA62C3.3, bA62C3.4
Gene Description
nucleolar protein 8
Omim ID
611534Gene Ontology
HyperlinkGene Summary
NOL8 binds Ras-related GTP-binding proteins (see MIM 608267) and plays a role in cell growth (Sekiguchi et al., 2004 [PubMed 14660641]).[supplied by OMIM
Other Designations
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Interactome
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Disease
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