SUMO2/3 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SUMO2/3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human SUMO2/3.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The SUMO2/3 polyclonal antibody (Cat # PAB2408) is used in Western blot to detect SUMO2/3 in HeLa cell lysate.Western Blot
COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels) . Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels : cell lysates probed by western blot (WB) with an anti-ERM antibody. Center panels : cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB with SUMO1 polyclonal antibody (Cat # PAB2402) . Bottom panels : cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with SUMO2/3 polyclonal antibody (Cat # PAB2408) . (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the SUMO2/3 polyclonal antibody (Cat # PAB2408) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — SUMO3
Entrez GeneID
6612Protein Accession#
P55854 (Gene ID : 6612);P61956 (Gene ID : 6613)Gene Name
SUMO3
Gene Alias
SMT3A, SMT3H1, SUMO-3
Gene Description
SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
Omim ID
602231Gene Ontology
HyperlinkGene Summary
SUMO proteins, such as SUMO3, and ubiquitin (see MIM 191339) posttranslationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (Su and Li, 2002 [PubMed 12383504]).[supplied by OMIM
Other Designations
OTTHUMP00000115275|SMT3 suppressor of mif two 3 homolog 1|small ubiquitin-like modifier protein 3
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Gene Info — SUMO2
Entrez GeneID
6613Protein Accession#
P55854 (Gene ID : 6612);P61956 (Gene ID : 6613)Gene Name
SUMO2
Gene Alias
HSMT3, MGC117191, SMT3B, SMT3H2
Gene Description
SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
Omim ID
603042Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
SMT3 suppressor of mif two 3 homolog 2|sentrin 2|small ubiquitin-like modifier 2, isoform a
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Interactome
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Disease
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Publication Reference
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SUMO modification of the Ets-related transcription factor ERM inhibits its transcriptional activity.
Degerny C, Monte D, Beaudoin C, Jaffray E, Portois L, Hay RT, de Launoit Y, Baert JL.
The Journal of Biological Chemistry 2005 Apr; 280(26):24330.
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SUMO modification of the Ets-related transcription factor ERM inhibits its transcriptional activity.
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